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Comment
. 2017 Oct;3(10):765-766.
doi: 10.1038/s41477-017-0025-5.

Seeing is believing

Tamar Azoulay-Shemer  1 , Po-Kai Hsu  2 , Julian I Schroeder  3
Affiliations
Comment

Seeing is believing

Tamar Azoulay-Shemer et al. Nat Plants. 2017 Oct.

Abstract

Gibberellins (GAs) control key growth and developmental processes in plants. Real-time monitoring of GA concentrations in living tissues is critical for understanding the actions of this hormone class. A first-generation optogenetic GA-nano-indicator now illuminates the effects of GA levels on cell length and light signalling.

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Conflict of interest statement

Competing interests

The authors declare no competing financial interests.

Figures

Fig. 1
Fig. 1. Real-time monitoring of GA concentrations in individual cells in plant tissues
The nano-reporter GPS1 targeted to nuclei (nlsGPS1) is used in plant hypocotyls (top and middle) and in roots (bottom) to monitor GA concentrations with cellular resolution. The pseudo-coloured calibration bar (nlsGPS1 emission ratio) indicates nuclear GA levels. Note that the dark-grown seedling (left, top) shows higher GA concentrations in hypocotyl cells than the light-grown seedling (middle), consistent with GA stimulation of hypocotyl growth. GPS1-FRET ratios in root tips indicate a GA gradient (bottom) that positively correlates with root cell length. Fluorescence images reproduced from ref. .
See this image and copyright information in PMC

Comment on

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