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Review
. 2017 Oct 3;16(1):168.
doi: 10.1186/s12934-017-0785-7.

Methods for genetic transformation of filamentous fungi

Dandan Li  1   2 , Yu Tang  3 , Jun Lin  4   5   6 , Weiwen Cai  7   8
Affiliations
Review

Methods for genetic transformation of filamentous fungi

Dandan Li et al. Microb Cell Fact. .

Abstract

Filamentous fungi have been of great interest because of their excellent ability as cell factories to manufacture useful products for human beings. The development of genetic transformation techniques is a precondition that enables scientists to target and modify genes efficiently and may reveal the function of target genes. The method to deliver foreign nucleic acid into cells is the sticking point for fungal genome modification. Up to date, there are some general methods of genetic transformation for fungi, including protoplast-mediated transformation, Agrobacterium-mediated transformation, electroporation, biolistic method and shock-wave-mediated transformation. This article reviews basic protocols and principles of these transformation methods, as well as their advantages and disadvantages.

Keywords: Agrobacterium-mediated transformation; Biolistic method; Electroporation; Filamentous fungi; Protoplast-mediated transformation; Shock-wave-mediated transformation.

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Figures

Fig. 1
Fig. 1
Basic steps of the protoplast-mediated transformation
Fig. 2
Fig. 2
The basic steps of the Agrobacterium–mediated transformation
See this image and copyright information in PMC

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