Measurement of dark adaptometry during ISCEV standard flash electroretinography

Abstract

Purpose: Dark adaptometry (DA) is a sensitive test for diagnosis of retinal disease. Patients requiring flash electroretinograms (ERGs) benefit from DA for proper diagnosis. Our purpose was to develop a DA test to be administered during the 20-min dark adaptation period of the flash ERG ( www.iscev.org ) using alternating red and blue stimuli to bias responses in favor of cones (red) and rods (blue).

Methods: An ERG Ganzfeld was used to measure DA in 21 normal subjects and 21 patients with retinal disease. Subjects underwent 75 s of preadaptation followed by 20 min of DA using an adaptive staircase to detect alternating flashes of red and blue light. Normative red and blue DA was compared to patients at the 10-min midpoint and 20-min final threshold.

Results: At 15 min, normals and patients showed asymptotic plateaus; hence, final thresholds were achieved within 15 min for parameters of this study. In RP and allied conditions (n = 11), 100% showed significantly elevated blue DA thresholds (> 2SD above normal) at 10 and 20 min. In cone diseases, 80% showed abnormal red DA at 10 min and 30% at 20 min, indicating that delayed adaptation is more typical of cone disease.

Conclusions: Two-color DA is expediently measured during the DA phase of ERGs providing clinical data which separate rod and cone dysfunction critical for accurate diagnosis of various retinal diseases. It provides an additional benchmark for monitoring function over time and invaluable guidance for patients functioning in dimly lit settings.

Keywords: Dark adaptometry; ERG; Electroretinography; Retinal disease; Retinitis pigmentosa.