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. 2017 Nov:12:1261-1269.
doi: 10.2217/fmb-2017-0080. Epub 2017 Oct 5.

Emergence of ISAba1 harboring carbapenem-resistant Acinetobacter baumannii isolates in Pakistan

Mohsin Khurshid  1   2 , Muhammad Hidayat Rasool  1 , Usman Ali Ashfaq  3 , Bilal Aslam  1 , Muhammad Waseem  1
Affiliations

Emergence of ISAba1 harboring carbapenem-resistant Acinetobacter baumannii isolates in Pakistan

Mohsin Khurshid et al. Future Microbiol. 2017 Nov.

Abstract

Aim: The ISAba1 insertion sequence supports the overexpression of various oxacillinases genes by providing promoter and therefore, confers carbapenem resistance. The study aimed to determine the clonal diversity, genetic attributes of carbapenem resistance and the role of ISAba1 in the expression of oxacillinases genes among Acinetobacter baumannii clinical isolates.

Materials & methods: PCR was performed to amplify various antimicrobial resistance determinants and ISAba1 insertion elements in 137 A. baumannii clinical isolates.

Results: The bla OXA51 and bla OXA23-like genes and ISAba1 upstream to these genes were found in almost all multidrug-resistant isolates belonging to multiple clones.

Conclusion: This is the first comprehensive study from Pakistan that highlighted the emergence of ISAba1 harboring carbapenems resistant A. baumannii stains in tertiary care hospitals.

Keywords: Acinetobacter baumannii; ISAba1; insertion sequence; oxacillinases.

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