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. 2017 Oct;25(4):804-811.
doi: 10.1016/j.jfda.2016.12.006. Epub 2017 Feb 16.

Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling

Kai-Di Hsu  1 , Shu-Pei Wu  2 , Shin-Ping Lin  1 , Chi-Chin Lum  2 , Kuan-Chen Cheng  1   2   3
Affiliations

Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling

Kai-Di Hsu et al. J Food Drug Anal. 2017 Oct.

Abstract

Extracellular polysaccharide (EPS) is one of the major bioactive ingredients contributing to the health benefits of Ganoderma spp. In this study, response surface methodology was applied to determine the optimal culture conditions for EPS production of Ganoderma formosanum. The optimum medium composition was found to be at initial pH 5.3, 49.2 g/L of glucose, and 4.9 g/L of yeast extract by implementing a three-factor-three-level Box-Behnken design. Under this condition, the predicted yield of EPS was up to 830.2 mg/L, which was 1.4-fold higher than the one from basic medium (604.5 mg/L). Furthermore, validating the experimental value of EPS production depicted a high correlation (100.4%) with the computational prediction response model. In addition, the percentage of β-glucan, a well-recognized bioactive polysaccharide, in EPS was 53±5.5%, which was higher than that from Ganoderma lucidum in a previous study. Moreover, results of monosaccharide composition analysis indicated that glucose was the major component of G. formosanum EPS, supporting a high β-glucan percentage in EPS. Taken together, this is the first study to investigate the influence of medium composition for G. formosanum EPS production as well as its β-glucan composition.

Keywords: Ganoderma formosanum; extracellular polysaccharide; medium composition; response surface methodology; β-glucan.

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Conflict of interest statement

Conflicts of interest

All authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Three-dimensional surface and contour plots of three factors on EPS production. The effect of (A) initial pH and glucose, (B) initial pH and yeast extract, and (C) glucose and yeast extract on EPS yield. EPS = extracellular polysaccharide; Glc = glucose; YE = yeast extract.
Figure 2
Figure 2
Effect of initial pH and medium composition on the morphology of mycelial pellets. Glc = glucose; YE = yeast extract.
See this image and copyright information in PMC

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