On the interactions among zinc availability and responses to ozone stress in durum wheat seedlings

Abstract

Seedlings of durum wheat [Triticum turgidum subsp. durum (Desf.) Husn] were exposed to zinc nutrition and to ozone (O₃) in a factorial combination: adequate (+Zn treatment) or no Zn (-Zn) in the nutrient solution, followed by exposure to either ozone-free air (filtered air, FA) or to 150 nL L^-1 ozone (O₃) for 4 h. Although omitting Zn from the nutrient solution failed to impose a genuine Zn deficiency, -Zn*FA durum wheat seedlings showed a typical deficiency behaviour, i.e. Zn mobilisation from root to shoot. Such inter-organ Zn redistribution, however, did not occur in -Zn*O₃ plants. Exposure to each stress singly decreased the activity and the protein amount of foliar plasma membrane H⁺-ATPase, but not stress combination, which even increased the H⁺-ATPase expression with respect to control. In the -Zn*O₃ plants, moreover, the foliar activities of the plasma membrane-bound NAD(P)H-dependent superoxide synthase and of Cu,Zn-superoxide dismutase, and the transcripts abundance of the luminal binding protein and of the protein disulphide isomerase, were also stimulated. It is proposed that, even in the absence of actual Zn starvation, the perception of deficiency conditions could trigger changes in redox homoeostasis at the plasma membrane level, helpful in compensating an O₃-dependent oxidative damage.

Keywords: Durum wheat; Plasma membrane; Redox activation; Surface ozone; Triticum turgidum subsp. durum (Desf.) Husn; Zinc.