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. 1988 Sep;85(18):6711-5.
doi: 10.1073/pnas.85.18.6711.

Acetyladenylate plays a role in controlling the direction of flagellar rotation

A J Wolfe  1 , M P Conley, H C Berg
Affiliations

Acetyladenylate plays a role in controlling the direction of flagellar rotation

A J Wolfe et al. Proc Natl Acad Sci U S A. 1988 Sep.

Abstract

Cells of Escherichia coli deleted for genes that code for the transducers and all the known cytoplasmic Che proteins except CheY responded reversibly to the addition of acetate by spinning their flagellar motors clockwise. By varying growth conditions and using metabolic inhibitors and mutants deficient in acetate metabolism, this effect was shown to require acetate-CoA synthetase [acetate:CoA ligase (AMP-forming); EC 6.2.1.1], an enzyme that catalyzes the formation of acetyl-CoA from acetate by an acetyladenylate intermediate. A mutant deficient in this enzyme but retaining the chemotaxis genes was deficient for chemotaxis. Thus, acetyladenylate appears to play a role in generating clockwise rotation at the level of CheY or the motor.

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References

    1. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5056-60 - PubMed
    1. J Gen Microbiol. 1973 Jan;74(1):77-91 - PubMed
    1. J Bacteriol. 1987 Mar;169(3):1307-14 - PubMed
    1. Biochem Biophys Res Commun. 1988 Mar 15;151(2):891-6 - PubMed
    1. Cell. 1987 Jun 5;49(5):579-81 - PubMed

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