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. 2017 Oct 10;7(1):12726.
doi: 10.1038/s41598-017-12939-7.

High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax

Duílio M Z de A Silva  1 , Ricardo Utsunomia  2 , Francisco J Ruiz-Ruano  3 , Sandro Natal Daniel  4 , Fábio Porto-Foresti  4 , Diogo Teruo Hashimoto  5 , Claudio Oliveira  2 , Juan Pedro M Camacho  3 , Fausto Foresti  2
Affiliations

High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax

Duílio M Z de A Silva et al. Sci Rep. .

Erratum in

Abstract

The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Examples of the chromosome distribution patterns of the SatDNAs found in A. paranae: non-clustered (a) and clustered (b). Each cell is shown with satDNA FISH (red) merged with DAPI (upper panel) and with satDNA FISH (lower panel). Note that the ApaSat36-21 does not show any FISH signal (non-clustered pattern). Bar = 10 μm.
Figure 2
Figure 2
Comparison of repeat unit length (RUL) between the satDNAs found in A. paranae according to their chromosome distribution pattern.
Figure 3
Figure 3
Mitotic metaphase cells of A. paranae (a), A. fasciatus (b) and A. bockmanni (c) showing the chromosome distribution of the ApaSat20-18 satDNA. The FISH signals are shown in red and are merged with DAPI in the upper panel. Bar = 10 μm.
Figure 4
Figure 4
Mitotic metaphase cells of A. paranae (a), A. fasciatus (b) and A. bockmanni (c) showing the chromosome distribution of the ApaSat02-236 satDNA. The FISH signals are shown in red and are merged with DAPI in the upper panel. Bar = 10 μm.
Figure 5
Figure 5
Distribution of satDNAs on the B chromosomes of A. paranae (BpM), A. fasciatus (BfMa) and A. bockmanni (BbM). The red numbers indicate catalog number for each satDNA. DAPI-stained chromosomes and satDNA hybridization patterns are displayed side-by-side for each B-variant. Note that all satDNAs showing signals on BpM and BbM chromosomes show symmetric distribution, whereas only five of them are symmetric in the BfMa chromosome.
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