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. 2017 Sep 26:8:1811.
doi: 10.3389/fmicb.2017.01811. eCollection 2017.

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Lagoon and Soil Matrices Associated with a Piggery Environment: Comparison of Expression Profiles

Anne-Laure Vivant  1   2 , Jeremy Desneux  1   2 , Anne-Marie Pourcher  1   2 , Pascal Piveteau  3
Affiliations

Transcriptomic Analysis of the Adaptation of Listeria monocytogenes to Lagoon and Soil Matrices Associated with a Piggery Environment: Comparison of Expression Profiles

Anne-Laure Vivant et al. Front Microbiol. .

Abstract

Understanding how Listeria monocytogenes, the causative agent of listeriosis, adapts to the environment is crucial. Adaptation to new matrices requires regulation of gene expression. To determine how the pathogen adapts to lagoon effluent and soil, two matrices where L. monocytogenes has been isolated, we compared the transcriptomes of L. monocytogenes CIP 110868 20 min and 24 h after its transfer to effluent and soil extract. Results showed major variations in the transcriptome of L. monocytogenes in the lagoon effluent but only minor modifications in the soil. In both the lagoon effluent and in the soil, genes involved in mobility and chemotaxis and in the transport of carbohydrates were the most frequently represented in the set of genes with higher transcript levels, and genes with phage-related functions were the most represented in the set of genes with lower transcript levels. A modification of the cell envelop was only found in the lagoon environment. Finally, the differential analysis included a large proportion of regulators, regulons, and ncRNAs.

Keywords: Listeria monocytogenes; RNAseq; lagoon effluent; microcosms; pig manure; soil; transcriptome.

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Figures

Figure 1
Figure 1
Principal component analysis of Listeria monocytogenes transcriptomes after 20 min and 24 h of incubation in lagoon effluent and soil extract.
Figure 2
Figure 2
Venn diagrams of genes showing significant differences after 20 min and 24 h. (A) Genes with higher transcript levels in the lagoon effluent, (B) genes with lower transcript levels in the lagoon effluent, (C) genes with higher transcript levels in the soil extract, (D) genes with lower transcript levels in the soil extract.
Figure 3
Figure 3
Number of genes in the lagoon effluent and in the soil extract, distributed in the most important functional categories.
See this image and copyright information in PMC

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