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. 2018:1664:79-86.
doi: 10.1007/978-1-4939-7268-5_7.

DIGE-Based Phosphoproteomic Analysis

Taras Stasyk  1 , Lukas Alfons Huber  2
Affiliations

DIGE-Based Phosphoproteomic Analysis

Taras Stasyk et al. Methods Mol Biol. 2018.

Abstract

Here, we describe the detailed step-by-step protocol for detection of phosphoproteins in two-dimensional difference gel electrophoresis (DIGE) gels. A standard DIGE protocol is combined with subsequent post-staining with phosphospecific fluorescent dye. The combination of these two methods complements DIGE-based proteome profiling by fluorescence detection of phosphoproteins in the same gel providing additional possibility for sensitive and accurate quantification of the differentially regulated phosphoproteins in biological samples.

Keywords: 2D-DIGE; Phosphoprotein; Phosphoproteomics.

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