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Case Reports
. 2017 Jun 14;4(6):e005099.
doi: 10.1099/jmmcr.0.005099. eCollection 2017 Jun.

Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19

Angela Saupe  1 , Birgit Edel  1 , Wolfgang Pfister  1 , Bettina Löffler  1 , Ralf Ehricht  2 , Jürgen Rödel  1
Affiliations
Case Reports

Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19

Angela Saupe et al. JMM Case Rep. .

Abstract

Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.

Keywords: STEC infection; antibiotics; azithromycin; diarrhoea.

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References

    1. Fruth A, Prager R, Tietze E, Rabsch W, Flieger A. Molecular epidemiological view on Shiga toxin-producing Escherichia coli causing human disease in Germany: diversity, prevalence, and outbreaks. Int J Med Microbiol. 2015;305:697–704. doi: 10.1016/j.ijmm.2015.08.020. - DOI - PubMed
    1. Fasel D, Mellmann A, Cernela N, Hächler H, Fruth A, et al. Hemolytic uremic syndrome in a 65-year-old male linked to a very unusual type of stx2e- and eae-harboring O51:H49 Shiga toxin-producing Escherichia coli. J Clin Microbiol. 2014;52:1301–1303. doi: 10.1128/JCM.03459-13. - DOI - PMC - PubMed
    1. Friesema IH, Keijzer-Veen MG, Koppejan M, Schipper HS, van Griethuysen AJ, et al. Hemolytic uremic syndrome associated with Escherichia coli O8:H19 and Shiga toxin 2f gene. Emerg Infect Dis. 2015;21:168–169. doi: 10.3201/eid2101.140515. - DOI - PMC - PubMed
    1. Lienemann T, Salo E, Rimhanen-Finne R, Rönnholm K, Taimisto M, et al. Shiga toxin-producing Escherichia coli serotype O78:H- in family, Finland, 2009. Emerg Infect Dis. 2012;18:577–581. doi: 10.3201/eid1804.111310. - DOI - PMC - PubMed
    1. Mellmann A, Harmsen D, Cummings CA, Zentz EB, Leopold SR, et al. Prospective genomic characterization of the German enterohemorrhagic Escherichia coli O104:H4 outbreak by rapid next generation sequencing technology. PLoS One. 2011;6:e22751. doi: 10.1371/journal.pone.0022751. - DOI - PMC - PubMed

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