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. 2017 Oct;4(5):309-314.
doi: 10.1093/rb/rbx026. Epub 2017 Sep 25.

Synthesis and characterization of collagen/PLGA biodegradable skin scaffold fibers

Alireza Sadeghi-Avalshahr  1 , Samira Nokhasteh  1 , Amir Mahdi Molavi  1 , Mohammad Khorsand-Ghayeni  1 , Meysam Mahdavi-Shahri  1
Affiliations

Synthesis and characterization of collagen/PLGA biodegradable skin scaffold fibers

Alireza Sadeghi-Avalshahr et al. Regen Biomater. 2017 Oct.

Abstract

The aim of this study is to investigate the applicability of poly(lactic-co-glycolic acid) (PLGA)/collagen composite scaffold for skin tissue engineering. PLGA and collagen were dissolved in HFIP as a common solvent and fibrous scaffolds were prepared by electrospinning method. The scaffolds were characterized by scanning electron microscopy (SEM), FTIR spectroscopy, mercury porosimetry, tensile strength, biocompatibility assays and Biodegradation. Cytotoxicity and cell adhesion were tested for two cell line groups, human dermal fibroblast (HDF) and human keratinocyte (HaCat). SEM images showed appropriate cell adhesion to the scaffold for both cell lines. MTT assays indicated that the cell viability of HDF cells increased with time, but the number of HaCat cells decreased after 14 days. The ultimate tensile strength was suitable for skin substitute application, but its elongation at break was rather low. For successful clinical application of the PLGA/collagen scaffold, some properties especially mechanical strain needs to be improved.

Keywords: biocompatibility; composite scaffold; skin cells; tissue engineering.

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Figures

Figure 1
Figure 1
SEM images of collagen/PLGA composite fibers (a) 10 000×, (b) 20 000×, (scale bar shows 2 μm); (c) distribution of fiber diameters; (d) porosimetry results of collagen/PLGA
Figure 2
Figure 2
FTIR analysis of collagen, PLGA and PLGA/collagen
Figure 3
Figure 3
The stress-elongation curve for PLGA/collagen scaffold
Figure 4
Figure 4
Weight loss of composite fibers
Figure 5
Figure 5
SEM images of cell adhesion (a, b) HDF cell line and (c, d) HaCat cell line
Figure 6
Figure 6
Quantitative evaluation of (a) HDF and (b) HaCat cytotoxicity (*P < 0.05)
See this image and copyright information in PMC

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