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. 2018:1689:157-166.
doi: 10.1007/978-1-4939-7380-4_14.

Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers

Teodora Ribarska  1 , Gregor D Gilfillan  2
Affiliations

Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers

Teodora Ribarska et al. Methods Mol Biol. 2018.

Abstract

ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.

Keywords: HTS; Micro-ChIP; N-ChIP; NGS; Next-generation sequencing.

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