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. 2017 Oct 4:6:104.
doi: 10.1186/s13756-017-0257-1. eCollection 2017.

Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food

Farhad Safarpoor Dehkordi  1 , Hasan Gandomi  1 , Afshin Akhondzadeh Basti  1 , Ali Misaghi  1 , Ebrahim Rahimi  2
Affiliations

Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food

Farhad Safarpoor Dehkordi et al. Antimicrob Resist Infect Control. .

Abstract

Background: Pathogenic biotypes of the Methicillin-resistant Staphylococcus aureus (MRSA) strains are considered to be one of the major cause of food-borne diseases in hospitals. The present investigation was done to study the pattern of antibiotic resistance and prevalence of antibiotic resistance genes of different biotypes of the MRSA strains isolated from various types of hospital food samples.

Methods: Four-hundred and eighty-five raw and cooked hospital food samples were cultured and MRSA strains were identified using the oxacillin and cefoxitin disk diffusion tests and mecA-based PCR amplification. Isolated strains were subjected to biotyping and their antibiotic resistance patterns were analyzed using the disk diffusion and PCR methods.

Results: Prevalence of S. aureus and MRSA were 9.69 and 7.62%, respectively. Meat and chicken barbecues had the highest prevalence of MRSA. Prevalence of bovine, ovine, poultry and human-based biotypes in the MRSA strains were 8.10, 8.10, 32.43 and 48.64%, respectively. All of the MRSA strains recovered from soup, salad and rice samples were related to human-based biotypes. MRSA strains harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), erythromycin (89.18%) and trimethoprim-sulfamethoxazole (83.78%). TetK (72.97%), ermA (72.97%), msrA (64.86%) and aacA-D (62.16%) were the most commonly detected antibiotic resistance genes.

Conclusions: Pattern of antibiotic resistance and also distribution of antibiotic resistance genes were related to the biotype of MRSA strains. Presence of multi-drug resistance and also simultaneous presence of several antibiotic resistance genes in some MRSA isolates showed an important public health issue Further researches are required to found additional epidemiological aspects of the MRSA strains in hospital food samples.

Keywords: Antimicrobial resistance; Biotypes; Hospital food; Methicillin-resistant Staphylococcus aureus.

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Conflict of interest statement

Ethics approval and consent to participate

The study was approved by the Ethical Council of Research of the Faculty of Veterinary Medicine University of Tehran, Tehran, Iran (Consent Ref Number 7507001/6/36). Verification of this research project and the licenses related to sampling process were approved by the Prof. Afshin Akhondzadeh Basti and Prof. Ebrahim Rahimi (Approval Ref Number FHQC 2016/10).

Consent for publication

There was no consent for publication.

Competing interests

The authors declare that they have no competing interests.

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