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. 1988 Nov;85(21):7849-51.
doi: 10.1073/pnas.85.21.7849.

Functional reconstitution of a proton-translocating system responsive to fusicoccin

P Aducci  1 , A Ballio, J P Blein, M R Fullone, M Rossignol, R Scalla
Affiliations

Functional reconstitution of a proton-translocating system responsive to fusicoccin

P Aducci et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

Crude fusicoccin binding proteins and a partially purified plasma membrane H+-transporting ATPase (EC 3.6.1.34), both solubilized from maize tissues, were simultaneously inserted into liposomes by the freeze-thaw method. ATP-driven intravesicular acidification in the proteoliposomes, measured by the fluorescence quenching of the dye 9-amino-6-chloro-2-methoxyacridine, markedly increased upon addition of fusicoccin to the reconstituted system. This effect could not be observed when binding sites and ATPase preparations were separately reconstituted into the proteoliposomes, thus demonstrating that fusicoccin binding to its receptor is a prerequisite for ATPase stimulation.

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