doi: 10.1016/0735-0651(88)90005-2.
Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer
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- PMID: 2903843
- DOI: 10.1016/0735-0651(88)90005-2
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Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer
Gene Anal Tech.
1988 Sep-Oct.
Abstract
A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.
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