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. 1988 Sep-Oct;5(5):105-7.
doi: 10.1016/0735-0651(88)90005-2.

Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer

J Hanish  1 , M McClelland
Affiliations

Activity of DNA modification and restriction enzymes in KGB, a potassium glutamate buffer

J Hanish et al. Gene Anal Tech. 1988 Sep-Oct.

Abstract

A single buffer consisting primarily of potassium and glutamate is shown to be suitable for activity of almost all restriction endonucleases, DNA methylases, and many other DNA-modifying enzymes. The composition of this buffer differs substantially from buffers currently in use for these enzymes.

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