doi: 10.1016/0735-0651(88)90003-9.
Rapid DNA purification for restriction fragment length polymorphism analysis
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- PMID: 2903845
- DOI: 10.1016/0735-0651(88)90003-9
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Rapid DNA purification for restriction fragment length polymorphism analysis
Gene Anal Tech.
1988 Sep-Oct.
Abstract
This paper describes a method for isolation of DNA from blood samples involving a rapid chemical disintegration of proteins with 8 M urea and with a minimum of exposure to phenol. The DNA is further desalted and purified on Sephadex G-25 prepacked disposable columns. DNA isolated in this way was pure enough to be immediately cleaved by restriction enzymes.
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