Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2017:2017:6496570.
doi: 10.1155/2017/6496570. Epub 2017 Sep 6.

MicroRNAs as Biomarkers in Thyroid Carcinoma

Marilena Celano  1 , Francesca Rosignolo  2 , Valentina Maggisano  1 , Valeria Pecce  2 , Michelangelo Iannone  3 , Diego Russo  1 , Stefania Bulotta  1
Affiliations
Review

MicroRNAs as Biomarkers in Thyroid Carcinoma

Marilena Celano et al. Int J Genomics. 2017.

Abstract

Optimal management of patients with thyroid cancer requires the use of sensitive and specific biomarkers. For early diagnosis and effective follow-up, the currently available cytological and serum biomarkers, thyroglobulin and calcitonin, present severe limitations. Research on microRNA expression in thyroid tumors is providing new insights for the development of novel biomarkers that can be used to diagnose thyroid cancer and optimize its management. In this review, we will examine some of the methods commonly used to detect and quantify microRNA in biospecimens from patients with thyroid tumor, as well as the potential applications of these techniques for developing microRNA-based biomarkers for the diagnosis and prognostic evaluation of thyroid cancers.

PubMed Disclaimer

Figures

Figure 1
Figure 1
miRNA detection workflow. miRNAs can be isolated from different biospecimens. To isolate miRNAs, widely used methods are chemical and column-based techniques. After quantification step, samples are ready for miRNA profiling. Among widely used techniques, there are four established methods: microarray, quantitative PCR (qRT-PCR), massive parallel sequencing (RNA-seq), and digital PCR (dPCR). The sensitivity and specificity are classified as follows: + (low), ++ (moderate), +++ (high). FFPE: formalin-fixed paraffin embedded; FNA: fine-needle aspiration.
See this image and copyright information in PMC

References

    1. Kitahara C. M., Sosa J. A. The changing incidence of thyroid cancer. Nature Reviewes Endocrinology. 2016;12(11):646–653. doi: 10.1038/nrendo.2016.110. - DOI - PMC - PubMed
    1. Cabanillas M. E., McFadden D. G., Durante C. Thyroid cancer. Lancet. 2016;388(10061):2783–2795. doi: 10.1016/S0140-6736(16)30172-6. - DOI - PubMed
    1. Schlumberger M., Lacroix L., Russo D., Filetti S., Bidart J. M. Defects in iodide metabolism in thyroid cancer and implications for the follow-up and treatment of patients. Nature Clinical Practice Endocrinology & Metabolism. 2007;3(3):260–269. doi: 10.1038/ncpendmet0449. - DOI - PubMed
    1. Puxeddu E., Durante C., Avenia N., Filetti S., Russo D. Clinical implications of BRAF mutation in thyroid carcinoma. Trends in Endocrinology and Metabolism. 2008;19(4):138–145. doi: 10.1016/j.tem.2007.12.003. - DOI - PubMed
    1. Xing M. Molecular pathogenesis and mechanisms of thyroid cancer. Nature Reviews Cancer. 2013;13(3):184–199. doi: 10.1038/nrc3431. - DOI - PMC - PubMed