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. 2017 Nov;14(11):1087-1089.
doi: 10.1038/nmeth.4467. Epub 2017 Oct 16.

Rapid nonlinear image scanning microscopy

Ingo Gregor  1   2 , Martin Spiecker  1 , Roman Petrovsky  3 , Jörg Großhans  3 , Robert Ros  4   5   6 , Jörg Enderlein  1   2
Affiliations

Rapid nonlinear image scanning microscopy

Ingo Gregor et al. Nat Methods. 2017 Nov.

Abstract

Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

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