Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction

Carmen Garcia-Jares¹, Marta Sanchez-Nande², Juan Pablo Lamas³, Marta Lores⁴

Affiliations

¹ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. carmen.garcia.jares@usc.es.
² Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. martasancheznande@gmail.com.
³ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. juanpablo.lamas@usc.es.
⁴ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. marta.lores@usc.es.

PMID: 29039745
PMCID: PMC5746754
DOI: 10.3390/bioengineering4040087

Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction

Carmen Garcia-Jares et al. Bioengineering (Basel). 2017.

. 2017 Oct 17;4(4):87.

doi: 10.3390/bioengineering4040087.

Authors

Carmen Garcia-Jares¹, Marta Sanchez-Nande², Juan Pablo Lamas³, Marta Lores⁴

Affiliations

¹ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. carmen.garcia.jares@usc.es.
² Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. martasancheznande@gmail.com.
³ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. juanpablo.lamas@usc.es.
⁴ Laboratorio de Investigación y Desarrollo de Soluciones Analíticas (LIDSA), Departamento de Química Analítica, Nutrición y Bromatología, Universidade de Santiago de Compostela, Facultad de Quimica, Avda das Ciencias s/n, Campus Vida, E-15782 Santiago de Compostela, Spain. marta.lores@usc.es.

PMID: 29039745
PMCID: PMC5746754
DOI: 10.3390/bioengineering4040087

Abstract

Camellia (genus of flowering plants of fam. Theaceae) is one of the main crops in Asia, where tea and oil from leaves and seeds have been utilized for thousands of years. This plant is excellently adapted to the climate and soil of Galicia (northwestern Spain) and northern Portugal where it is grown not only as an ornamental plant, but to be evaluated as a source of bioactive compounds. In this work, the main fatty acids were extracted from Camellia seeds of four varieties of Camellia: sasanqua, reticulata, japonica and sinensis, by means of matrix-solid phase dispersion (MSPD), and analyzed by gas chromatography (GC) with MS detection of the corresponding methyl esters. MSPD constitutes an efficient and greener alternative to conventional extraction techniques, moreover if it is combined with the use of green solvents such as limonene. The optimization of the MSPD extraction procedure has been conducted using a multivariate approach based on strategies of experimental design, which enabled the simultaneous evaluation of the factors influencing the extraction efficiency as well as interactions between factors. The optimized method was applied to characterize the fatty acids profiles of four Camellia varieties seeds, allowing us to compare their fatty acid composition.

Keywords: Camellia seeds; GC-MS; fatty acids; limonene; matrix solid-phase dispersion.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
Scheme of the Matrix Solid Phase Dispersion Procedure for *Camellia* Seeds.

**Figure 2**
Mean plots of the main factors studied in the multi-factor categorical design for oleic acid: (a) extraction solvent; (b) dispersant type; (c) eluting volume.

**Figure 3**
Interaction plots between the adsorbent type and the extraction solvent for the target fatty acids: (a) palmitic; (b) linoleic; (c) oleic; and (d) stearic.

**Figure 4**
Total ion chromatogram (TIC) showing the main fatty acids of Camellia japonica seeds.

See this image and copyright information in PMC

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Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction

Affiliations

Profiling the Fatty Acids Content of Ornamental Camellia Seeds Cultivated in Galicia by an Optimized Matrix Solid-Phase Dispersion Extraction

Authors

Affiliations

Abstract

Conflict of interest statement

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