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. 2017 Oct;14(4):2976-2982.
doi: 10.3892/etm.2017.4924. Epub 2017 Aug 11.

Elevation of PTPN1 promoter methylation is a significant risk factor of type 2 diabetes in the Chinese population

Qing Huang  1 , Liyuan Han  2 , Yanfen Liu  2 , Changyi Wang  3 , Donghui Duan  2 , Nanjia Lu  2 , Kaiyue Wang  2 , Lu Zhang  2 , Kaibo Gu  2 , Shiwei Duan  2 , Yifeng Mai  1
Affiliations

Elevation of PTPN1 promoter methylation is a significant risk factor of type 2 diabetes in the Chinese population

Qing Huang et al. Exp Ther Med. 2017 Oct.

Abstract

The present study aimed to investigate the contribution of DNA methylation of the protein tyrosine phosphatase, non-receptor type 1 (PTPN1) gene to the susceptibility to type 2 diabetes (T2D). Peripheral blood mononuclear cells (PBMCs) were collected from 97 patients with T2D and 97 age- and gender-matched controls. DNA methylation of the PTPN1 gene promoter was evaluated by bisulfite pyrosequencing. Independent sample t-tests were used to compare the differences in the PTPN1 promoter and other phenotypes between the patients with T2D and the controls. The results indicated a significant correlation between PTPN1 promoter methylation and the risk of T2D. Additionally, a breakdown analysis by gender revealed that PTPN1 methylation was associated with an increased risk of T2D in females. Furthermore, low-density lipoprotein (r=-0.183, P=0.046) and total cholesterol (r=-0.310, P=0.001) were inversely associated with PTPN1 methylation in females. In conclusion, the results indicate that elevated PTPN1 promoter methylation is a risk factor for T2D in the female Chinese population.

Keywords: methylation; non-receptor type 1; promoter; protein tyrosine phosphatase; type 2 diabetes.

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Figures

Figure 1.
Figure 1.
Pairwise correlation among eight methylated cytosine-guanine dinucleotide sites in the PTPN1 gene promoter. PTPN1, protein tyrosine phosphatase, non-receptor type 1; CpG, cytosine-guanine dinucleotide site; UTR, untranslated region.
Figure 2.
Figure 2.
Comparison of the mean DNA methylation levels of the protein tyrosine phosphatase, non-receptor type 1 gene promoter between patients with type 2 diabetes and controls.
Figure 3.
Figure 3.
Correlation analyses between the mean protein tyrosine phosphatase, non-receptor type 1 DNA methylation and clinical phenotypes. Correlation analyses between (A) the mean PTPN1 DNA methylation and TC level, and (B) the mean PTPN1 DNA methylation and LDL level. TC, total cholesterol; LDL, low-density lipoprotein.
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