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. 2017 Oct;14(4):3369-3374.
doi: 10.3892/etm.2017.5012. Epub 2017 Aug 24.

Screening of differentially expressed proteins in psoriasis vulgaris by two-dimensional gel electrophoresis and mass spectrometry

Yinan Dai  1 , Qingrui Zhang  1   2 , Yi Jiang  2 , Lu Yin  1 , Xiaodong Zhang  1 , Yang Chen  2 , Xinze Cai  2
Affiliations

Screening of differentially expressed proteins in psoriasis vulgaris by two-dimensional gel electrophoresis and mass spectrometry

Yinan Dai et al. Exp Ther Med. 2017 Oct.

Abstract

The aim of the present study was to elucidate differentially expressed proteins in lesional tissues of psoriasis vulgaris (PV) and normal tissues. Lesional skin tissues were collected from PV patients, along with normal skin tissues from healthy individuals. The protein content of the samples was extracted and then separated by two-dimensional gel electrophoresis (2-DGE). Any proteins that were differentially expressed in the lesional skin of PV patients compared with the healthy controls were analyzed by mass spectrometry and bioinformatics. In the stratum corneum and dermis of PV patients, the total number of proteins identified by 2-DGE was 1,969±21 and 1,928±49, respectively. Of these, 30 proteins were differentially expressed in the PV patients, of which 14 were identified as: Type 1 keratin cytoskeleton proteins (including K1C10, K1C14, K1C15 and K1C16); the type 2 keratin cytoskeleton protein, K2C1; actin-associated proteins (including ARP3, ACTA and ACTBM); prohibitin; heat shock proteins (HSPB1 and CH60); centrosome protein, CP135; and membrane associated proteins (including ANXA4 and ANXA5). The differential expression of protein between PV lesions and normal tissue can be considered as pathological biomarker. Elucidating the abnormal regulation of these proteins can provide mechanism of the development of PV and may contribute to significant approaches for PV treatments.

Keywords: differentially expressed proteins; mass spectrometry; psoriasis vulgaris; two-dimensional gel electrophoresis.

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Figures

Figure 1.
Figure 1.
Microscope images of psoriasis vulgaris lesions (A) prior to and (B) following sectioning, observed with methylene blue staining (magnification, ×10).
Figure 2.
Figure 2.
Gel map of stratum corneum in patients with PV using (A) Cy3 and (B) Cy5 staining. (C) Fluorescent gel map of stratum corneum in patients with PV. PV, psoriasis vulgaris.
Figure 3.
Figure 3.
Location and three-dimension representation of CP135 in the gel. Location of CP-135 in the gel from (A) the control group dermis and (B) the psoriasis group's dermis. Three-dimensional images of CP-135 in the gels from (C) the control group dermis and (D) the psoriasis group dermis.
See this image and copyright information in PMC

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