Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Oct 18;7(1):13436.
doi: 10.1038/s41598-017-13723-3.

Dissecting the Underlying Pharmaceutical Mechanism of Chinese Traditional Medicine Yun-Pi-Yi-Shen-Tong-Du-Tang Acting on Ankylosing Spondylitis through Systems Biology Approaches

Duoli Xie  1 , Lin Huang  1 , Guanghui Zhao  2 , Yiran Yu  1 , Jiawei Gao  1 , Haichang Li  1 , Chengping Wen  3
Affiliations

Dissecting the Underlying Pharmaceutical Mechanism of Chinese Traditional Medicine Yun-Pi-Yi-Shen-Tong-Du-Tang Acting on Ankylosing Spondylitis through Systems Biology Approaches

Duoli Xie et al. Sci Rep. .

Abstract

Traditional Chinese Medicine (TCM) has been served as complementary medicine for Ankylosing Spondylitis (AS) treatment for a long time. Yun-Pi-Yi-Shen-Tong-Du-Tang (Y-Y-T) is a novel empirical formula designed by Prof. Chengping Wen. In this study, a retrospective investigation supported efficacy of Y-Y-T and then we deciphered the underlying molecular mechanism of the efficacy. Herbal ingredients and targeting proteins were collected from TCMID. PPI networks were constructed to further infer the relationship among Y-Y-T, drugs used for treating AS, differentially expressed genes of AS patients and AS disease proteins. Finally, it was suggested that TLR signaling pathway and T cell receptor signaling pathway may involve in the biological processes of AS progression and contribute to the curative effect and proteins such as JAK2, STAT3, HSP90AA1, TNF and PTEN were the key targets. Our systemic investigation to infer therapeutic mechanism of Y-Y-T for AS treatment provides a new insight in understanding TCM pharmacology.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
The relationship between herbs. Same ingredients shared between 5 herbs, the width of the lines represent the number of same ingredients shared between herbs.
Figure 2
Figure 2
The relationship between ingredients. Sharing targets more than 3 between ingredients were showed up in the network. The width of the lines represent the number of shared targets between ingredients.
Figure 3
Figure 3
Classification of the formula’s top 20 enriched Go terms. The different colors represent different functional categories and the lower levels affiliation to upper levels.
Figure 4
Figure 4
The overlaps between formula targets, disease genes, drug targets and DEGs. Blue: disease genes; Yellow: DEGs; pink: Y-Y-T targets; green: drug targets.
Figure 5
Figure 5
A cluster PPI network of the Y-Y-T and disease genes. Green: formula targets; yellow: disease genes; red: formula and disease genes.
Figure 6
Figure 6
A cluster PPI network of the Y-Y-T formula and DEGs. Green: formula targets; red: formula targets and up-regulated DEGs; yellow: formula targets and down -regulated DEGs; blue: up-regulated DEGs; purple: down -regulated DEGs.
Figure 7
Figure 7
A cluster PPI network of the Y-Y-T and drug targets. Green: formula targets; red: formula targets and drug targets. Y-Y-T; Yun-Pi-Yi-Shen-Tong-Du-Tang.
See this image and copyright information in PMC

References

    1. Duran A, et al. Fecal calprotectin is associated with disease activity in patients with ankylosing spondylitis. Bosnian journal of basic medical sciences / Udruzenje basicnih mediciniskih znanosti = Association of Basic Medical Sciences. 2016;16:71–74. - PMC - PubMed
    1. Osman, M. S. & Maksymowych, W. P. An update on the use of tumor necrosis factor alpha inhibitors in the treatment of ankylosing spondylitis. Expert review of clinical immunology (2016). - PubMed
    1. Zhou YY, Lin JH, Huang RY, He YT. Treatment of Ankylosing Spondylitis With a Bushen-Qiangdu-Zhilv Decoction: A Case Report With a 3-year Follow-up. Alternative therapies in health and medicine. 2016;22(Suppl 1):36–40. - PubMed
    1. Machado, N. P. et al. Clinical characteristics and frequency of TLR4 polymorphisms in Brazilian patients with ankylosing spondylitis. Revista brasileira de reumatologia (2016). - PubMed
    1. Bae, J. M., Choo, J. Y., Kim, K. J. & Park, K. S. Association of inflammatory bowel disease with ankylosing spondylitis and rheumatoid arthritis: A nationwide population-based study. Modern rheumatology / the Japan Rheumatism Association, 1–6 (2016). - PubMed

Publication types

MeSH terms