Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Dec;107(6 Pt 1):2075-86.
doi: 10.1083/jcb.107.6.2075.

Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes

S Froshauer  1 , J Kartenbeck, A Helenius
Affiliations

Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes

S Froshauer et al. J Cell Biol. 1988 Dec.

Abstract

Using morphological and cell biological techniques, we have shown that the RNA replicase of Semliki Forest and Sindbis virus (two closely related alphaviruses) is located in complex ribonucleoprotein structures associated with the cytoplasmic surface of modified secondary lysosomes and endosomes. These nucleoprotein complexes often form a bridge between the membrane of the endocytic vacuole and the rough endoplasmic reticulum where the synthesis of the structural proteins of these viruses occurs. The results suggest that these cytopathic vacuoles constitute sites not only for viral RNA synthesis, but also for translation of structural proteins, and for the assembly of nucleocapsids.

PubMed Disclaimer

References

    1. Virology. 1967 May;32(1):128-43 - PubMed
    1. J Virol. 1968 Nov;2(11):1326-38 - PubMed
    1. Int Rev Cytol. 1969;26:235-302 - PubMed
    1. Ann Med Exp Biol Fenn. 1969;47(4):235-48 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

Publication types

MeSH terms