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. 2017 Dec:107:59-62.
doi: 10.1016/j.tube.2017.08.003. Epub 2017 Aug 10.

Detection of mycobacterial infection in non-human primates using the Xpert MTB/RIF molecular assay

Duanne Alves da Silva  1 , Amanda M Rêgo  1 , Nicole V Ferreira  1 , Manoela A S de Andrade  1 , Aline R Campelo  1 , Paulo César S Caldas  1 , Márcia Aparecida S Pereira  1 , Paulo Redner  1 , Lucindo C de Pina  1 , Felipe C Resende  2 , Thalita A Pissinatti  2 , Claudia A A Lopes  2 , Tatiana Kugelmeier  2 , Javier A S Perea  2 , Igo V de Souza  2 , Fábio A da Silva  2 , Carla F Campos  2 , Fátima C O Fandinho Montes  1 , L Caetano M Antunes  3
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Detection of mycobacterial infection in non-human primates using the Xpert MTB/RIF molecular assay

Duanne Alves da Silva et al. Tuberculosis (Edinb). 2017 Dec.

Abstract

Tuberculosis is a major public health concern, and diagnostic strategies applied to animal populations are scarce. As part of ongoing efforts to control tuberculosis dissemination at our animal facility, two non-human primates (NHP, Saimiri sciureus) presenting cutaneous lesions were examined for mycobacterial infection. Both animals tested positive for acid-fast bacilli and Mycobacterium tuberculosis using a molecular assay (IS6110 PCR). Animals were euthanized and several samples were tested for M. tuberculosis using the Xpert MTB/RIF assay. Many samples were positive for M. tuberculosis and rifampicin resistance, and some produced mycobacterial growth. Oral swabs from cage mates were then tested with Xpert MTB/RIF, and the majority tested positive for M. tuberculosis and rifampicin resistance, and produced growth in culture. To our knowledge, this is the first report of multidrug-resistant mycobacterial infection in NHP. Additionally, our data shows that the Xpert MTB/RIF assay can be useful as a screening tool for tuberculosis infection in NHP.

Keywords: Diagnosis; Resistance; Tuberculosis; Zoonosis.

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