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. 2018 Mar;43(3):259-265.
doi: 10.1139/apnm-2017-0334. Epub 2017 Oct 20.

Investigation of the C-3-epi-25(OH)D3 of 25-hydroxyvitamin D3 in urban schoolchildren

Samantha E Berger  1 , Maria I Van Rompay  1 , Catherine M Gordon  2 , Elizabeth Goodman  3 , Misha Eliasziw  4 , Michael F Holick  5 , Jennifer M Sacheck  1
Affiliations

Investigation of the C-3-epi-25(OH)D3 of 25-hydroxyvitamin D3 in urban schoolchildren

Samantha E Berger et al. Appl Physiol Nutr Metab. 2018 Mar.

Abstract
in English, French

The physiological relevance C-3 epimer of 25-hydroxyvitamin D (3-epi-25(OH)D) is not well understood among youth. The objective of this study was to assess whether demographic/physiologic characteristics were associated with 3-epi-25(OH)D3 concentrations in youth. Associations between 3-epi-25(OH)D3 and demographics and between 3-epi-25(OH)D3, total 25-hydroxyvitamin (25(OH)D) (25(OH)D2 + 25(OH)D3), total cholesterol, high-density lipoprotein, low-density lipoprotein, and triglycerides were examined in racially/ethnically diverse schoolchildren (n = 682; age, 8-15 years) at Boston-area urban schools. Approximately 50% of participants had detectable 3-epi-25(OH)D3 (range 0.95-3.95 ng/mL). The percentage of 3-epi-25(OH)D3 of total 25(OH)D ranged from 2.5% to 17.0% (median 5.5%). Males were 38% more likely than females to have detectable 3-epi-25(OH)D3 concentrations. Both Asian and black race/ethnicity were associated with lower odds of having detectable 3-epi-25(OH)D3 compared with non-Hispanic white children (Asian vs. white, odds ratio (OR) 0.28, 95% confidence interval (CI) 0.14-0.53; black vs. white, OR 0.38, 95%CI 0.23-0.63, p < 0.001). Having an adequate (20-29 ng/mL) or optimal (>30 ng/mL) 25(OH)D concentration was associated with higher odds of having detectable 3-epi-25(OH)D3 than having an inadequate (<20 ng/mL) concentration (OR 4.78, 95%CI 3.23-6.94 or OR 14.10, 95%CI 7.10-28.0, respectively). There was no association between 3-epi-25(OH)D3 and blood lipids. However, when considering 3-epi-25(OH)D3 as a percentage of total 25(OH)D, total cholesterol was lower in children with percent 3-epi-25(OH)D3 above the median (mean difference -7.1 mg/dL, p = 0.01). In conclusion, among schoolchildren, sex, race/ethnicity, and total serum 25(OH)D concentration is differentially associated with 3-epi-25(OH)D. The physiological relevance of 3-epi-25(OH)D3 may be related to the 3-epi-25(OH)D3 as a percentage of total 25(OH)D and should be considered in future investigations.

La pertinence physiologique de l’épimère C-3 de la 25-hydroxyvitamine D (« 3-epi-25(OH)D ») chez les jeunes n’est pas bien établie. Cette étude se propose de vérifier si des caractéristiques démographiques/physiologiques sont associéesà la concentration de 3-epi-25(OH)D chez les jeunes. On examine, chez des enfants d’âge scolaire de diverses races/ethnies (n = 682, âge : 8–15 ans) dans la région urbaine de Boston, la relation entre 3-epi-25(OH)D3 et des variables démographiques, entre 3-epi-25(OH)D3 et 25-hydroxyvitamine (« 25(OH)D ») (25(OH)D2 + 25(OH)D3) totale, cholestérol total, lipoprotéines de haute densité, lipoprotéine de faible densité et les triglycérides. Environ 50 % des enfants présentent une concentration détectable de 3-epi-25(OH)D3 (écart : 0,95−3,95 ng/mL). Le pourcentage de 3-epi-25(OH)D3 parrapportà25(OH)Dtotalevariede2,5à17,0%(médiane:5,5%). Lesgarçonssont38%plusà mêmequelesfillesdeprésenterune concentration détectable de 3-epi-25(OH)D3. Les enfants de race/ethnie noire et asiatique ont une plus faible probabilité de présenter une concentration détectable de 3-epi-25(OH)D3 comparativement aux enfants de race blanche non hispaniques (Asiatiques vs Blancs, rapport des cotes (RC) 0,28, intervalle de confiance (IC) 95 % 0,14–0,53; Noirs vs Blancs, RC 0,38, IC 95 % 0,23−0,63, p < 0,001). Un taux adéquat (20–29 ng/mL) ou optimal (>30 ng/mL) de 25(OH)D est associéà une probabilité plus élevée de présenter une concentration détectable de 3-epi-25(OH)D3 comparativementà un taux inadéquat (<20 ng/mL) : RC 4,78, IC 95 % 3,23–6,94 ou RC 14,10, IC 95 % 7,10–28,0, respectivement. Il n’y a pas de relation entre 3-epi-25(OH)D3 et les lipides sanguins. Toutefois, en considérant 3-epi-25(OH)D3 en pourcentage de 25(OH)D totale, les enfants présentant un taux de 3-epi-25(OH)D3 supérieurà la médiane (différence moyenne :−7,1 mg/dL, p = 0,01) ont un plus faible taux de cholestérol total. Conclusion. Chez les enfants d’âge scolaire, le sexe, la race/ethnie et la concentration sérique de 25(OH)D totale sont différemment associés au taux de 3-epi-25(OH)D. La pertinence physiologique de 3-epi-25(OH)D3 pourrait être envisagée en pourcentage de 25(OH)D totale et devrait être prise en considération dans les études ultérieures. [Traduit par la Rédaction]

Keywords: 25-hydroxyvitamin D3; 25-hydroxyvitamine D3; C-3 epimer; Daily D Health Study; vitamin D; vitamine D; épimère C-3; étude de la vitamine D journalière sur la santé.

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Figures

Fig. 1.
Fig. 1.
(A) Distribution of 3-epimer of 25-hydroxyvitamin D (3-epi-25(OH)D3) among racially/ethnically diverse schoolchildren. (B) Distribution of percentage of 3-epi(25(OH)D3. One child not included in the figure had a 3-epimer concentration of 12.3 ng/mL and a percentage of 3-epi-25(OH)D of 37.6%.
Fig. 2.
Fig. 2.
(A) Scatter plot of 3-epimer of 25-hydroxyvitamin D (3-epi-25(OH)D3) and 25-hydroxyvitamin D (25(OH)D3). (B) Scatterplot of percentage of 3-epi-25(OH)D3 and 25(OH)D3. Individual with 3-epimer of 12.3 ng/mL was not included in graph.
See this image and copyright information in PMC

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