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. 2018 Feb:37:1-5.
doi: 10.1016/j.mcp.2017.10.002. Epub 2017 Oct 18.

Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses

Feng Cong  1 , Yujun Zhu  1 , Xiangnan Liu  2 , Xiuzhen Li  1 , Meili Chen  3 , Ren Huang  3 , Pengju Guo  4
Affiliations

Development of an xTAG-multiplex PCR array for the detection of four avian respiratory viruses

Feng Cong et al. Mol Cell Probes. 2018 Feb.

Abstract

Acute respiratory tract infections are of paramount importance in the poultry industry. We developed an xTAG bead assay for the simultaneous detection and discrimination of avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bronchitis virus (IBV) and infectious laryngotracheitis virus (ILTV). The assay lacked nonspecific reactions with other common avian viruses and the limit of detection was 6.75 × 102- 3.52 × 103copies/μL. We examined 60 clinical specimens and found 18 positive for respiratory viruses. Our result demonstrated that xTAG-multiplex PCR method is a high-throughput, rapid, specific and sensitive assay for use in epidemiological studies and clinical detection of avian respiratory pathogens.

Keywords: AIV; IBV; ILTV; Luminex; NDV; xTAG-multiplex PCR.

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