Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 May 10;4(2):26.
doi: 10.3390/vetsci4020026.

Prevalence and Multilocus Genotyping Analysis of Cryptosporidium and Giardia Isolates from Dogs in Chiang Mai, Thailand

Sahatchai Tangtrongsup  1   2 , A Valeria Scorza  3 , John S Reif  4 , Lora R Ballweber  5 , Michael R Lappin  6 , Mo D Salman  7
Affiliations

Prevalence and Multilocus Genotyping Analysis of Cryptosporidium and Giardia Isolates from Dogs in Chiang Mai, Thailand

Sahatchai Tangtrongsup et al. Vet Sci. .

Abstract

The occurrence and zoonotic potential of Cryptosporidium spp. and Giardia duodenalis isolated from dogs in Chiang Mai, Thailand were determined. Fecal samples were collected from 109 dogs between July and August 2008. Cryptosporidium spp. infection was determined by immunofluorescent assay (IFA), PCR assays that amplify Cryptosporidium heat-shock protein 70 kDa (hsp70), and two PCR assays that amplify a small subunit-ribosomal RNA (SSU-rRNA). Giardiaduodenalis infection was identified using zinc sulfate centrifugal flotation, IFA, and four PCR assays that amplify the Giardia glutamate dehydrogenase (gdh), beta-giardin (bg), and generic and dog-specific assays of triosephosphate isomerase (tpi) genes. Overall prevalence of Cryptosporidium spp. and G.duodenalis was 31.2% and 45.9%, respectively. Sequence analysis of 22 Cryptosporidium-positive samples and 21 Giardia-positive samples revealed the presence of C.canis in 15, and C. parvum in 7, G. duodenalis Assemblage C in 8, D in 11, and mixed of C and D in 2 dogs. Dogs in Chiang Mai were commonly exposed to Cryptosporidium spp. and G. duodenalis. Cryptosporidium parvum can be isolated from the feces of dogs, and all G. duodenalis assemblages were dog-specific. Dogs could be a reservoir for a zoonotic Cryptosporidium infection in humans, but further studies will be required to determine the clinical and zoonotic importance.

Keywords: Chiang Mai; Cryptosporidium; Giardia; Thailand; dogs.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Thompson R.C., Smith A. Zoonotic enteric protozoa. Vet. Parasitol. 2011;182:70–78. doi: 10.1016/j.vetpar.2011.07.016. - DOI - PubMed
    1. Scorza V., Tangtrongsup S. Update on the diagnosis and management of Cryptosporidium spp. infections in dogs and cats. Top. Companion Anim. Med. 2010;25:163–169. doi: 10.1053/j.tcam.2010.07.007. - DOI - PubMed
    1. Tangtrongsup S., Scorza V. Update on the diagnosis and management of Giardia spp. infections in dogs and cats. Top. Companion Anim. Med. 2010;25:155–162. doi: 10.1053/j.tcam.2010.07.003. - DOI - PubMed
    1. Feng Y., Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin. Microbiol. Rev. 2011;24:110–140. doi: 10.1128/CMR.00033-10. - DOI - PMC - PubMed
    1. Slapeta J. Cryptosporidiosis and Cryptosporidium species in animals and humans: A thirty colour rainbow? Int. J. Parasitol. 2013;43:957–970. doi: 10.1016/j.ijpara.2013.07.005. - DOI - PubMed

LinkOut - more resources