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. 2016 Jun 1;3(2):9.
doi: 10.3390/vetsci3020009.

Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

Florian R L Meyer  1 , Ingrid Walter  2
Affiliations

Establishment and Characterization of New Canine and Feline Osteosarcoma Primary Cell Lines

Florian R L Meyer et al. Vet Sci. .

Abstract

Osteosarcomas are the most abundant form of bone malignancies in multiple species. Canine osteosarcomas are considered a valuable model for human osteosarcomas because of their similar features. Feline osteosarcomas, on the other hand, are rarely studied but have interesting characteristics, such as a better survival prognosis than dogs or humans, and less likelihood of metastasis. To enable experimental approaches to study these differences we have established five new canine osteosarcoma cell lines out of three tumors, COS_1186h, COS_1186w, COS_1189, and COS_1220, one osteosarcoma-derived lung metastasis, COS_1033, and two new feline osteosarcoma cell lines, FOS_1077 and FOS_1140. Their osteogenic and neoplastic origin, as well as their potential to produce calcified structures, was determined by the markers osteocalcin, osteonectin, tissue unspecific alkaline phosphatase, p53, cytokeratin, vimentin, and alizarin red. The newly developed cell lines retained most of their markers in vitro but only spontaneously formed spheroids produced by COS_1189 showed calcification in vitro.

Keywords: cat; cell culture; dog; osteosarcoma.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Phase contrast images showing growth morphology of (a) COS_1033 at P4; (b) COS_1189 at P7; (c) COS_1220 at P4; (d) FOS_1140 at P4; (e) COS_1186w at P3; (f) COS_1186h at P4; and (g) FOS_1077 at P4. Scale bars represent 100 µm for (a–d) and 200 µm for (e–g).
Figure 2
Figure 2
Alizarin red staining of (a) a tumor section of 1189; (b) a monolayer cell culture sample of COS_1189; and (c) a spheroid of COS_1189. Scale bar represents 20 µm.
Figure 3
Figure 3
Immunofluorescence staining with anti-vimentin and anti-cytokeratin (inserts) of the canine tumor 1033 (a) and the resulting cell culture (b) as well as the feline tumor 1077 (c) and its resulting cell culture (d). Scale bar represents 25 µm.
Figure 4
Figure 4
Immunhistochemistry and immunofluorescence of canine tumor 1220 (a,e,i) and its cell line (b,f,j), and the feline tumor 1077 (c,g,k) and its cell line (d,h,l) showing osteonectin (a–d), osteocalcin (e–h), and tissue unspecific alkaline phosphatase (i–l). Scale bar represents 10 µm.
Figure 5
Figure 5
Imunhistological staining of p53 in the canine tumor 1189 (a) and feline osteosarcoma 1140 (c), and the cell lines COS_1189h (b) and FOS_1140 (d) derived from these tumors. Scale bar represents 20 µm.
See this image and copyright information in PMC

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