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. 2015 Jun 11;2(2):84-96.
doi: 10.3390/vetsci2020084.

Intranuclear Inclusions in Renal Tubular Epithelium in Immunodeficient Mice Stain with Antibodies for Bovine Papillomavirus Type 1 L1 Protein

Elizabeth McInnes  1 , Mark Bennett  2 , Mandy O'Hara  3 , Lorna Rasmussen  4 , Peony Fung  5 , Philip Nicholls  6 , Michael Slaven  7 , Robert Stevenson  8
Affiliations

Intranuclear Inclusions in Renal Tubular Epithelium in Immunodeficient Mice Stain with Antibodies for Bovine Papillomavirus Type 1 L1 Protein

Elizabeth McInnes et al. Vet Sci. .

Abstract

The kidneys from six immunodeficient mice examined by Cerberus Sciences and the Animal Resources Centre, displayed karyomegaly with pale eosinophilic, intranuclear inclusions upon histopathological examination. Electron microscopy performed on kidney tissue from 5/6 mice demonstrated margination of the chromatin in large nuclei. Laboratory tests were used to detect nucleic acid of papillomaviruses, polyomaviruses, circoviruses and anelloviruses (4/6 mice), a specific PCR was used to detect murine polyomavirus (1/6), and a panel of serological tests was used to detect seroconversion to major murine pathogens (1/6). All molecular and serological tests were negative. Immunohistochemistry using polyclonal anti-bovine papillomavirus type 1 (BPV-1) L1 antibody, Camvir monoclonal anti-papillomavirus antibody (directed against the seven amino acids GFGAMDF found in human papillomavirus (HPV) 16 L1 protein), a commercially available mixture of two monoclonal antibodies, anti-BPV-1 L1/1H8 + Camvir antibodies, and a monoclonal anti-Hsc70 antibody revealed specific, positive staining of murine renal tubular epithelial intranuclear inclusions in 6/6 mice using the anti-BPV-1 L1 containing antibodies only. Methyl pyronin green, PAS and Feulgen histochemical reactions revealed that the intranuclear inclusions did not consist of RNA, DNA or carbohydrate. An immunohistochemical method now exists that can be used to confirm and evaluate suspected cases of murine inclusion body nephropathy.

Keywords: bovine papillomavirus type 1 L1 protein; intranuclear inclusions; mouse; renal tubular epithelium.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Renal cortical tubular intranuclear inclusion bodies (black arrowhead) from mouse F stained with haematoxylin and eosin. The black measurement bar represents 100 μm.
Figure 2
Figure 2
Chromatin displaced peripherally within affected nuclei, around abundant electron lucent material. Mouse A (lead citrate and uranyl acetate).
Figure 3
Figure 3
(a) Renal cortical tubular intranuclear inclusion bodies from mouse F stained with haematoxylin and eosin (b) Feulgen reaction, (c) Methyl Green Pyronin, (d) Periodic Acid Schiff’s reaction, (e) anti-Hsc70 monoclonal antibody, (f) polyclonal rabbit anti bovine papillomavirus type 1 L1 protein antibody, (g) a mixture of Camvir + monoclonal mouse anti bovine papillomavirus type 1 L1 protein antibody (the latter component’s epitope binding specificity is not known), (h) Camvir monoclonal antibody. The black arrows demonstrate the findings in the intranuclear inclusions.
See this image and copyright information in PMC

References

    1. Besselsen D.G., Franklin C.L., Livingston R.S., Riley L.K. Lurking in the shadows: Emerging rodent infectious diseases. ILAR J. 2008;49:277–290. doi: 10.1093/ilar.49.3.277. - DOI - PMC - PubMed
    1. Baze W.B., Steinbach T.J., Fleetwood M.L., Blanchard T.W., Barnhart K.F., McArthur M.J. Karyomegaly and intranuclear inclusions in the renal tubules of sentinel ICR mice (Mus musculus) Comp. Med. 2006;56:435–438. - PubMed
    1. Rector A., Tachezy R., Van Doorslaer K., MacNamara T., Burk R.D., Sundberg J.P., Van Ranst M. Isolation and cloning of a papillomavirus from a North American porcupine by using multiply primed rolling-circle amplification: The Erethizon dorsatum papillomavirus type 1. Virology. 2005;331:449–456. doi: 10.1016/j.virol.2004.10.033. - DOI - PubMed
    1. Bancroft J.D., Fredenburgh J.L., Grizzle W.E., Myers R.B. Proteins and nucleic acids. In: Bancroft J.D., Stevens M., editors. Theory and Practice of Histological Techniques. 5th ed. Churchill Livingstone; Edinburgh, UK: 2002. pp. 217–233.
    1. Australian Code for the Care and Use of Animals for Scientific Purposes. 8th ed. 2013. [(accessed on 5 June 2015)]. Available online: http://www.webcitation.org/6YwpUvaz3.

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