Ocular coherence tomography image data of the retinal laminar structure in a mouse model of oxygen-induced retinopathy

Abstract

The data presented in this article are related to the research paper entitled "Norrin treatment improves ganglion cell survival in an oxygen-induced model of retinal ischemia" (Dailey et al., 2017) [1] This article describes treatment with the human Norrin protein, an atypical Wnt-protein, to improve the survival of retinal ganglion cells in a murine model of Oxygen-Induced Retinopathy (OIR). That study utilized Optical coherence tomography (OCT) to visualize retinal layers at high resolution in vivo, and to quantify changes to nerve fiber layer thickness. Organization of the laminar structure of other retinal layers in this model in vivo, were not known because of uncertainties regarding potential artifacts during the processing of tissue for traditional histology. The OCT image data provided here shows researchers the retinal laminar structural features that exist in vivo in this popular mouse OIR model. Traditional H&E stained retinal tissue sections are also provided here for comparison.

Keywords: Mouse retina; Ocular coherence tomography; Oxygen-induced retinopathy; Retina; Retinal imaging.

Fig. 1

Retinal histology (hematoxylin and eosin staining) of the OIR retina. (A) Example of retinal morphology from a room air (normal) mouse, post-natal age 42 days (P42) and (B) vehicle-injected OIR mouse (P42) with zones of disruption in the laminar retinal architecture of the central retina (black arrows). (C) Zoomed view of the central retina from the same room air (normal) retinal section, showing the characteristically smaller and more densely stained nuclei of the photoreceptor layer. (D) Zoomed view of the disrupted central retina from the same vehicle-injected OIR mouse retina shows the location of dense photoreceptor nuclei (black arrows) with nuclei of the inner nuclear layer.

Fig. 2

SD-OCT horizontal B-scan data of the retinal laminar structure at ages P42 and P56 in the same OIR retinas. Horizontal B-scans in the superior retina (A) Scan of a vehicle-injected OIR eye at ages P42 and P56. (B) Scan of a Norrin-injected OIR eye at ages P42 and P56.