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. 1988:(19):175-8.

Studies on essential amino acid residues and functional regions of H+-ATPase (F0F1) from Escherichia coli by gene manipulation

T Noumi  1 , M Takeyama, M Kuki, M Maeda, M Futai
Affiliations
  • PMID: 2906427

Studies on essential amino acid residues and functional regions of H+-ATPase (F0F1) from Escherichia coli by gene manipulation

T Noumi et al. Nucleic Acids Symp Ser. 1988.

Abstract

We discussed application of in vitro mutagenesis on H+-ATPase (F0F1) of Escherichia coli. The oligonucleotide-directed site specific mutagenesis and construction of a set of truncated subunits were useful for identifying essential residues of beta subunit and a functional region of epsilon subunit, respectively, of this complicated membrane enzyme.

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