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. 2017 Nov;591(22):3781-3792.
doi: 10.1002/1873-3468.12889. Epub 2017 Nov 5.

Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster

Robert Gustafsson  1 , Ronnie P-A Berntsson  1   2 , Markel Martínez-Carranza  1 , Geniver El Tekle  1 , Richard Odegrip  1 , Eric A Johnson  3 , Pål Stenmark  1
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Free article

Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster

Robert Gustafsson et al. FEBS Lett. 2017 Nov.
Free article

Abstract

Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.

Keywords: TULIP; Crystallography; X-ray; botulinum neurotoxin; gene cluster.

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