Isolation versus Enrichment: dCLIP Enables Stringent Profiling of RNA-Binding Sites
- PMID: 29073369
- DOI: 10.1016/j.cels.2017.10.007
Isolation versus Enrichment: dCLIP Enables Stringent Profiling of RNA-Binding Sites
Abstract
Denaturing crosslinking and immunoprecipitation (dCLIP) enables stringent purification of ribonucleoprotein complexes to identify direct RNA targets of individual protein complex members.
Copyright © 2017 Elsevier Inc. All rights reserved.
Comment on
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Denaturing CLIP, dCLIP, Pipeline Identifies Discrete RNA Footprints on Chromatin-Associated Proteins and Reveals that CBX7 Targets 3' UTRs to Regulate mRNA Expression.Cell Syst. 2017 Oct 25;5(4):368-385.e15. doi: 10.1016/j.cels.2017.09.014. Cell Syst. 2017. PMID: 29073373 Free PMC article.
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