Basolateral amino acid and glucose transport by the intestine of the teleost, Anguilla anguilla

Abstract

1. D-glucose transport into BLMV was osmotically reactive, sodium independent, and inhibited by phloretin but not by phloridzin. 2. The survey of 6 L-amino acids identified three groups with respect to transfer across the basolateral cell border. Transport of proline and glutamate occurred by Na-dependent carriers and by apparent simple diffusion. Alanine, lysine and phenylalanine were transported by Na-independent carriers and apparent simple diffusion. Glycine transport was stimulated above apparent simple diffusion only by a simultaneous inwardly-directed Na gradient and outwardly-directed K gradient. 3. Only proline and glutamate demonstrated the ability to depolarize the membrane potential, consistent with Na-dependent rheogenic transport.