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. 2018:1698:275-284.
doi: 10.1007/978-1-4939-7428-3_17.

Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines

Divya S Vinjamur  1 , Daniel E Bauer  2   3
Affiliations

Growing and Genetically Manipulating Human Umbilical Cord Blood-Derived Erythroid Progenitor (HUDEP) Cell Lines

Divya S Vinjamur et al. Methods Mol Biol. 2018.

Abstract

The recently established human umbilical cord blood-derived erythroid progenitor (HUDEP) cell lines have equipped red blood cell researchers with valuable in vitro models of erythroid development. Of the three established HUDEP cell lines, HUDEP-2 cells express predominantly adult β-globin and most closely resemble adult erythroid cells. This chapter describes culture protocols for the maintenance and erythroid differentiation of HUDEP-2 cells. Methods to genetically manipulate HUDEP-2 cells using a CRISPR/Cas9 nuclease-based approach are also discussed.

Keywords: CRISPR/Cas9; Erythroid differentiation; Fetal hemoglobin; HUDEP; Hemoglobin switching; Immortalized erythroid cell line.

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