Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2017 Nov 7;114(45):11809-11811.
doi: 10.1073/pnas.1716866114. Epub 2017 Oct 24.

Helicase SPRNTing through the nanopore

Colleen C Caldwell  1 , Maria Spies  2
Affiliations
Comment

Helicase SPRNTing through the nanopore

Colleen C Caldwell et al. Proc Natl Acad Sci U S A. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Defining the HEL308–ssDNA translocation mechanism by SPRNT. (A) Schematic representation of the SPRNT experiment that utilizes HEL308 DNA helicase as the DNA translocating motor and the reengineered MspA porin as a biological nanopore. (B) Domain organization and motions of HEL308.
See this image and copyright information in PMC

Comment on

References

    1. Wu CG, Spies M. Overview: What are helicases? Adv Exp Med Biol. 2013;767:1–16. - PubMed
    1. Squires AH, Gilboa T, Torfstein C, Varongchayakul N, Meller A. Single-molecule characterization of DNA-protein interactions using nanopore biosensors. Methods Enzymol. 2017;582:353–385. - PubMed
    1. Laszlo AH, Derrrington IM, Gundlach JH. Subangstrom measurements of enzyme function using a biological nanopore, SPRNT. Methods Enzymol. 2017;582:387–414. - PMC - PubMed
    1. Craig JM, et al. Revealing dynamics of helicase translocation on single-stranded DNA using high-resolution nanopore tweezers. Proc Natl Acad Sci USA. 2017;114:11932–11937. - PMC - PubMed
    1. Kasianowicz JJ, Brandin E, Branton D, Deamer DW. Characterization of individual polynucleotide molecules using a membrane channel. Proc Natl Acad Sci USA. 1996;93:13770–13773. - PMC - PubMed

Publication types