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Review
. 2017:1035:31-48.
doi: 10.1007/978-3-319-67358-5_3.

Quantitative Live Cell FLIM Imaging in Three Dimensions

Alix Le Marois  1 , Klaus Suhling  2
Affiliations
Review

Quantitative Live Cell FLIM Imaging in Three Dimensions

Alix Le Marois et al. Adv Exp Med Biol. 2017.

Abstract

In this chapter, the concept of fluorescence lifetime and its utility in quantitative live cell imaging will be introduced, along with methods to record and analyze FLIM data. Relevant applications in 3D tissue and live cell imaging, including multiplexed FLIM detection, will also be detailed.

Keywords: FLIM data analysis; FLIM techniques; Multi-channel FLIM; Multi-modal FLIM; Optical sectioning; Time-correlated single photon counting (TCSPC).

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