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Comment
. 2017 Sep;6(Suppl 4):S462-S464.
doi: 10.21037/tau.2017.03.73.

Utility of testing sperm DNA fragmentation: an all-in-one diagnostic tool to address a multi-pronged clinical problem

D N Rao Veeramachaneni  1
Affiliations
Comment

Utility of testing sperm DNA fragmentation: an all-in-one diagnostic tool to address a multi-pronged clinical problem

D N Rao Veeramachaneni. Transl Androl Urol. 2017 Sep.
No abstract available

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Conflict of interest statement

Conflicts of Interest: The author has no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Transmission electron micrographs of constituents of equine seminal ejaculates. Impaired condensation of sperm nuclear chromatin associated with bacterial infection. (A) Bacteria embedded in degenerate membranous aggregates; (B) Incomplete nuclear condensation (arrow) and broken down chromatin (arrow heads); (C) Acrosomal swelling (arrow) and premature decondensation of chromatin; (D) Degenerate desquamated round spermatid and clumps of broken down chromatin (arrow heads). Panels E-H. Impaired condensation of sperm nuclear chromatin associated with abnormal proliferation of microtubules during spermiogenesis. The microtubular mass defect involves proliferation of abnormal microtubules from sites associated with manchette, a microtubular organelle important in shaping the spermatozoon, and is known to occur following chronic exposure to synthetic steroids; (E) Dispersed microtubule mass (arrow); (F-G) Microtubule masses disrupting nuclear condensation (asterisks) and marginated nuclear chromatin (arrows); (H) Degenerate desquamated round spermatid. Arrow points to malformed sperm with incomplete condensation of nucleus. Scale bars =1 µm.
Figure 2
Figure 2
Characterization of the pattern of SDF. (A) Photomicrograph of toluidine blue-stained equine sperm and clusters of broken down chromatin. (B-C) Electron micrographs of equine sperm with impaired condensation (B; asterisks) or premature decondensation of nuclear chromatin (C; arrow) showing broken down aggregates of chromatin (arrow heads). Scale bars = A: 10 µm; B-C: 1 µm.
See this image and copyright information in PMC

Comment in

  • All-round approach in diagnosis.
    Agarwal A, Cho CL, Esteves SC, Majzoub A. Agarwal A, et al. Transl Androl Urol. 2017 Sep;6(Suppl 4):S465-S467. doi: 10.21037/tau.2017.04.22. Transl Androl Urol. 2017. PMID: 29082940 Free PMC article. No abstract available.

Comment on

References

    1. Agarwal A, Majzoub A, Esteves SC, et al. Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Transl Androl Urol 2016;5:935-50. 10.21037/tau.2016.10.03 - DOI - PMC - PubMed
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    1. Veeramachaneni DN, Moeller CL, Pickett BW, et al. On processing and evaluation of equine seminal samples for cytopathology and fertility assessment: the utility of electron microscopy. J Equine Vet Sci 1993;13:207-15. 10.1016/S0737-0806(06)81013-0 - DOI

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