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Comment
. 2017 Sep;6(Suppl 4):S468-S470.
doi: 10.21037/tau.2017.03.78.

Eight tests for sperm DNA fragmentation and their roles in the clinic

W Steven Ward  1   2
Affiliations
Comment

Eight tests for sperm DNA fragmentation and their roles in the clinic

W Steven Ward. Transl Androl Urol. 2017 Sep.
No abstract available

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Conflict of interest statement

Conflicts of Interest: The author has no conflicts of interest to declare.

Figures

Figure 1
Figure 1
Sperm chromatin structure and SDF assays. (A) Intact sperm chromatin is condensed into protamine-bound toroids that are linked by short stretches of the chromatin and anchored to the nuclear matrix. TB, AB and CMA3 tests all assay DNA in its intact state; (B) the TUNEL assay also measures DNA damage in situ; (C) the SCSA and AO tests assay sperm chromatin from which the histones have been removed; (D) the Comet assay assesses DNA from which all the protamines and toroids have been removed. This image is modified from one by Gawecka, et al. (10). SCSA, sperm chromatin structure assay; TUNEL, Terminal deoxynucleotidyl transferase dUTP nick end labeling.
See this image and copyright information in PMC

Comment in

  • From bench to clinic.
    Agarwal A, Cho CL, Majzoub A, Esteves SC. Agarwal A, et al. Transl Androl Urol. 2017 Sep;6(Suppl 4):S471-S472. doi: 10.21037/tau.2017.04.23. Transl Androl Urol. 2017. PMID: 29082931 Free PMC article. No abstract available.

Comment on

References

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    1. Ward WS. Function of sperm chromatin structural elements in fertilization and development. Mol Hum Reprod 2010;16:30-6. 10.1093/molehr/gap080 - DOI - PMC - PubMed
    1. Hud NV, Downing KH. Cryoelectron microscopy of lambda phage DNA condensates in vitreous ice: the fine structure of DNA toroids. Proc Natl Acad Sci U S A 2001;98:14925-30. 10.1073/pnas.261560398 - DOI - PMC - PubMed

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