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Comment
. 2017 Sep;6(Suppl 4):S495-S500.
doi: 10.21037/tau.2017.07.20.

Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment

Donald P Evenson  1   2   3
Affiliations
Comment

Evaluation of sperm chromatin structure and DNA strand breaks is an important part of clinical male fertility assessment

Donald P Evenson. Transl Androl Urol. 2017 Sep.
No abstract available

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Conflict of interest statement

Conflicts of Interest: The author has no conflicts of interest to declare.

Figures

Figure 1
Figure 1
SCSA data for clinical report. (A) Raw data from a flow cytometer showing each of 5,000 sperm as a single dot on a scattergram. Y = native DNA stainability, X = fragmented DNA; high DNA stainability (HDS) sperm with uncondensed chromatin; (B) SCSAsoft® software conversion to: Y = total DNA stainability, X = DNA fragmentation index; (C) frequency histogram of data in middle panel. Three levels of DNA fragmentation: non-detectable, moderate level and high level. SCSA, Sperm Chromatin Structure Assay.
Figure 2
Figure 2
Odds of in vivo/IUI pregnancy vs. %DFI. The curve of decreasing odds with increasing %DFI is drawn from data on semen samples used for in vivo (n=732) (10,27,28) and IUI (n=387) (29) clinical studies. Vertical lines between 20–40%DFI indicate suggested clinical intervention thresholds from data (n≥1,500 semen samples) in ART clinical studies [e.g. (23,29,30)].
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References

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    1. Evenson DP. Sperm Chromatin Structure assay (SCSA®): Evolution from origin to clinical utility. In: A Zini A, Agarwal A. editors. A Clinician’s Guide to Sperm DNA and Chromatin Damage. New York: Springer Science, 2017.
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