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. 2017:2017:3921258.
doi: 10.1155/2017/3921258. Epub 2017 Aug 31.

Expression Levels and Clinical Significance of miR-21-5p, miR-let-7a, and miR-34c-5p in Laryngeal Squamous Cell Carcinoma

Massimo Re  1 , Giuseppe Magliulo  2 , Federico M Gioacchini  1 , Arisa Bajraktari  1 , Andrea Bertini  1 , Artan Çeka  3 , Corrado Rubini  4 , Luigi Ferrante  5 , Antonio D Procopio  3   6 , Fabiola Olivieri  3   6
Affiliations

Expression Levels and Clinical Significance of miR-21-5p, miR-let-7a, and miR-34c-5p in Laryngeal Squamous Cell Carcinoma

Massimo Re et al. Biomed Res Int. 2017.

Abstract

Objective: Altered microRNAs (miRNAs) expression has been found in many cancer types, including laryngeal squamous cell carcinoma (LSCC). The aim of this study was to determine the role and clinical value of three LSCC-related miRs, such as miR-21-5p, miR-let-7a, and miR-34c-5p in a homogeneous cohort of patients with primary LSCC treated by primary surgery.

Methods: Expression levels of miR-21-5p, miR-let-7a, and miR-34c-5p were detected in 43 pairs of LSCC and adjacent normal tissues by reverse-transcription quantitative PCR. Overall survival and disease-free survival were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using the Cox proportional hazard analysis.

Results: miR-21-5p is significantly upregulated, while miR-let-7a is significantly downregulated in LSCC tumor tissues compared with the corresponding adjacent normal tissues. The downregulation of miR-34c-5p expression significantly correlated with a shorter disease-free survival and, in the multivariate analysis, low miR-34c-5p expression was associated with an increased risk of recurrence.

Conclusions: miR-21-5p, miR-let-7a, and miR-34c-5p seem to play a critical role in LSCC carcinogenesis and might have a diagnostic and prognostic clinical value. The miR-let-7a levels could have a predictive role for lymph node metastases and miR-34c-5p might be a promising biomarker of patient outcome.

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Figures

Figure 1
Figure 1
miR-21-5p (a), miR-let7a (b), the miR-21-5p/miR-let7a ratio (c), and miR-34c-5p (d) relative expression in healthy, laryngeal samples and LSCC samples.
Figure 2
Figure 2
miR-let-7a and nodal involvement relative expression (N).
Figure 3
Figure 3
Sensitivity and specificity of miR-21-5p, miR-34c-5p, and miR-let7a (a) and miR-21-5p/miR-let7a, miR-21-5p/miR-34c-5p, and miR-34c-5p/miR-let7a (b) in discriminating healthy from LSCC samples, according to the Receiver Operating Characteristic (ROC) curve analysis.
Figure 4
Figure 4
Prognostic accuracy of miRs in laryngeal squamous carcinoma, according to ROC curve analysis.
Figure 5
Figure 5
Disease-free survival stratified for miR-34c-5p and pN, according to Kaplan–Meier estimates.
Figure 6
Figure 6
Survival function stratified for miR-34-5p and pN, according to Kaplan–Meier estimates.
See this image and copyright information in PMC

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