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. 2017 Oct;22(43):17-00032.
doi: 10.2807/1560-7917.ES.2017.22.43.17-00032.

VIM-1 carbapenemase-producing Escherichia coli isolated from retail seafood, Germany 2016

Nicole Roschanski  1 , Sebastian Guenther  1 , Thi Thu Tra Vu  2 , Jennie Fischer  3 , Torsten Semmler  4 , Stephan Huehn  2   5 , Thomas Alter  2 , Uwe Roesler  1
Affiliations

VIM-1 carbapenemase-producing Escherichia coli isolated from retail seafood, Germany 2016

Nicole Roschanski et al. Euro Surveill. 2017 Oct.

Erratum in

  • Author's correction for Euro Surveill. 2017;22(43).
    Eurosurveillance editorial team. Eurosurveillance editorial team. Euro Surveill. 2017 Nov;22(45):171109-c1. doi: 10.2807/1560-7917.ES.2017.22.45.171139c1. Euro Surveill. 2017. PMID: 29162203 Free PMC article. No abstract available.

Abstract

Carbapenems belong to the group of last resort antibiotics in human medicine. Therefore, the emergence of growing numbers of carbapenemase-producing bacteria in food-producing animals or the environment is worrying and an important concern for the public health sector. In the present study, a set of 45 Enterobacteriaceae isolated from German retail seafood (clams and shrimps), sampled in 2016, were investigated by real-time PCR for the presence of carbapenemase-producing bacteria. One Escherichia coli (ST10), isolated from a Venus clam (Ruditapes philippinarum) harvested in the Mediterranean Sea (Italy), contained the carbapenemase gene blaVIM-1 as part of the variable region of a class I integron. Whole-genome sequencing indicated that the integron was embedded in a Tn3-like transposon that also contained the fluoroquinolone resistance gene qnrS1. Additional resistance genes such as the extended-spectrum beta-lactamase blaSHV-12 and the AmpC gene blaACC-1 were also present in this isolate. Except blaACC-1, all resistance genes were located on an IncY plasmid. These results confirm previous observations that carbapenemase-producing bacteria have reached the food chain and are of increasing concern for public health.

Keywords: Enterobacteriaceae; beta-lactamases; multidrug resistance.

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Conflict of interest statement

Conflict of interest: None declared.

Figures

Figure
Figure
Comparison of the bla VIM-1-containing transposon region of the plasmids pOW16C2 isolated from Klebsiella pneumoniae derived from river water sampled in Switzerland and pE-124–4 isolated from Escherichia coli derived from retail seafood, Germany, 2016
See this image and copyright information in PMC

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