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. 2017 Apr 10:2017:1323802.
doi: 10.1155/2017/1323802. eCollection 2017.

Analytical Interference by Contrast Agents in Biochemical Assays

Sigrid Otnes  1 , Niels Fogh-Andersen  2 , Janne Rømsing  1 , Henrik S Thomsen  3
Affiliations

Analytical Interference by Contrast Agents in Biochemical Assays

Sigrid Otnes et al. Contrast Media Mol Imaging. .

Abstract

Objective: To provide a clinically relevant overview of the analytical interference by contrast agents (CA) in laboratory blood test measurements.

Materials and methods: The effects of five CAs, gadobutrol, gadoterate meglumine, gadoxetate disodium, iodixanol, and iomeprol, were studied on the 29 most frequently performed biochemical assays. One-day-old plasma, serum, and whole blood were spiked with doses of each agent such that the gadolinium agents and the iodine agents reached concentrations of 0.5 mM and 12 mg iodine/mL, respectively. Subsequently, 12 assays were reexamined using 1/2 and 1/4 of these CA concentrations. The results were assessed statistically by a paired Student's t-test.

Results: Iodixanol produced a negative interference on the bicarbonate (p = 0.011), lactate dehydrogenase (p < 0.0001), and zinc (p = 0.0034) assays and a positive interference on the albumin (p = 0.0062), calcium (p < 0.0001), ionized calcium (p = 0.0086), iron (p < 0.0001), and potassium (p = 0.0003) assays. Iomeprol produced a negative interference on the bicarbonate (p = 0.0057) and magnesium (p = 0.0001) assays and a positive interference on the calcium (p < 0.0001) and potassium (p = 0.0012) assays. Gadoxetate disodium produced a negative interference on the iron (p < 0.0001) and zinc (p < 0.0001) assays and a positive interference on the sodium (p = 0.032) assay.

Conclusion: CAs cause analytical interference. Attention should be given to the above-mentioned analyte-CA combinations when assessing laboratory blood test results obtained after CA administration.

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Figures

Figure 1
Figure 1
Interferogram presenting iodixanol and iomeprol interference in calcium assays (a), gadoxetate disodium interference in the iron (b) and zinc assays (c), and iomeprol interference in the magnesium assay (d). The error bars represent the SEM.∗p < 0.05 from Student's t-test, ∗∗p < 0.01 from Student's t-test, and ∗∗∗p < 0.001 from Student's t-test.
See this image and copyright information in PMC

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