Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jul;76(7):3174-8.
doi: 10.1073/pnas.76.7.3174.

Nucleoside triphosphate regeneration decreases the frequency of translation errors

P C Jelenc, C G Kurland

Nucleoside triphosphate regeneration decreases the frequency of translation errors

P C Jelenc et al. Proc Natl Acad Sci U S A. 1979 Jul.

Abstract

The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) reduced further the missense error frequency to the in vivo level as well as enhanced the extent of translation. The effect of nucleoside triphosphate regeneration was shown to be due primarily to the increase in the ratio of adenosine and guanosine triphosphates to their hydrolysis products and only marginally due to the increase in the absolute concentrations of the nucleoside triphosphates.

PubMed Disclaimer

References

    1. J Biol Chem. 1960 Mar;235:776-82 - PubMed
    1. Proc Natl Acad Sci U S A. 1962 Jan 15;48:94-104 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 Oct;52:988-96 - PubMed
    1. J Mol Biol. 1964 Jun;8:823-34 - PubMed
    1. Proc Natl Acad Sci U S A. 1964 May;51:883-90 - PubMed

MeSH terms

LinkOut - more resources