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Comparative Study
. 1979 Jul;76(7):3294-8.
doi: 10.1073/pnas.76.7.3294.

Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitro

Comparative Study

Moloney murine sarcoma virions synthesize full-genome-length double-stranded DNA in vitro

E W Benz Jr et al. Proc Natl Acad Sci U S A. 1979 Jul.

Abstract

Moloney murine sarcoma virus (MSV) virions incubated under optimal conditions were shown to support extensive synthesis of double-stranded DNA. The major product, a 5950-base-pair (6-kilobase-pair DNA) double-stranded DNA, was characterized by cleavage with restriction endonucleases and shown to contain a 600-nucleotide-long direct repeat at both ends of the MSV genome. Linear DNA molecules made in vivo shortly after infection were compared to the linear double-stranded DNA synthesized in vitro. The restriction maps of both viral DNA products were indistinguishable. The 600-base-pair repeat results in a progeny DNA molecule that is longer than the parental MSV genomic RNA. The generation of this repeat must involve a mechanism that allows the viral reverse transcriptase (RNA-dependent DNA nucleotidyltransferase) to copy 5'- and 3'-terminal genomic (+) strand sequences twice.

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References

    1. J Virol. 1978 Dec;28(3):810-8 - PubMed
    1. J Virol. 1978 May;26(2):479-84 - PubMed
    1. Cell. 1979 Jan;16(1):63-75 - PubMed
    1. Cell. 1979 Apr;16(4):863-74 - PubMed
    1. Cell. 1978 Mar;13(3):435-51 - PubMed

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