Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Nov 3;16(1):189.
doi: 10.1186/s12934-017-0805-7.

Kinetic modeling of Shewanella baltica KB30 growth on different substrates through respirometry

Juan Carlos Leyva-Díaz  1   2   3 , José Manuel Poyatos  1   2 , Paolo Barghini  3 , Susanna Gorrasi  3 , Massimiliano Fenice  4   5
Affiliations

Kinetic modeling of Shewanella baltica KB30 growth on different substrates through respirometry

Juan Carlos Leyva-Díaz et al. Microb Cell Fact. .

Abstract

Background: Shewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia). This strain is known for its ability to grow on a pool of different substrates, including carbohydrates, carboxylic and amino acids, and lipids. However, no data are available on its metabolic efficiency in relation to the use of different carbon sources typologies. This work represents the first attempt to characterize S. baltica by its heterotrophic kinetic performance.

Results: Growth and substrate consumption, during the biodegradation of sodium acetate, glucose, tween 80 and peptone, were analyzed through a respirometric method. To find the model best fitting the experimental data and to obtain the kinetic parameters, the equations of Monod, Moser, Contois and Tessier were applied. The kinetic behavior of S. baltica was fitted to Monod model for sodium acetate and tween 80, while it was adjusted to Contois model for glucose and peptone. In this regard, peptone was consumed faster than the other substrates, as indicated by the highest values of substrate degradation rate, which exceeded 60 mg O2 L-1 h-1.

Conclusions: Proteolytic metabolism was favored than lipidic and glucidic metabolism, which could contribute much more to mineralization and recycling of proteins than lipids and carbohydrates.

Keywords: Carbon utilization; Kinetics; Modeling; Shewanella baltica.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Schematic diagram of the bioreactor used in the respirometric experiments (a); evolution of dissolved oxygen (DO) (b) and schematic diagram of the assessment of the kinetic parameters for the biodegradation of different substrates by Shewanella baltica KB30: yield coefficient (Y), substrate concentration S, biomass concentration (X), oxygen consumption (OC), maximum specific growth rate (µm), empirical specific growth rate (µemp), dynamic oxygen uptake rate (Rs) and substrate half-saturation coefficient (Ks) (c)
Fig. 2
Fig. 2
Time course of dissolved oxygen (DO) in a typical respirometric experiment carried out with Shewanella baltica KB30 for the metabolization of the selected substrates: sodium acetate (a), glucose (b), tween 80 (c) and peptone (d)
Fig. 3
Fig. 3
Degrading ability of Shewanella baltica KB30 regarding different substrate categories (carbohydrates, carboxylic and amino acids, amine/amides and lipids), as previously determined by the Biolog Microarray system. Arrows’ thickness is proportional to the number of substrates used for each category
Fig. 4
Fig. 4
Substrate degradation rate (rsu) of Shewanella baltica KB30 during the respirometric experiments in relation to substrate concentration (S), measured as chemical oxygen demand in (mg O2 L−1), obtained by different models: Monod (a), Moser (b), Contois (c) and Tessier (d)
Fig. 5
Fig. 5
Cell growth rate (rx) of Shewanella baltica KB30 during the respirometric experiments in relation to substrate concentration (S), measured as chemical oxygen demand in (mg O2 L−1), obtained by different models: Monod (a), Moser (b), Contois (c) and Tessier (d)
See this image and copyright information in PMC

References

    1. Shewan JM, Hobbs G, Hodgkiss W. A determinative scheme for the identification of certain genera of Gram-negative bacteria with special reference to Pseudomonodaceae. J Appl Bacteriol. 1960;23:379–390. doi: 10.1111/j.1365-2672.1960.tb00211.x. - DOI
    1. Macdonell MT, Colwell RR. Phylogeny of the Vibrionaceae and recommendation for two new genera Listonella and Shewanella. Syst Appl Microbiol. 1985;6:171–182. doi: 10.1016/S0723-2020(85)80051-5. - DOI
    1. Ivanova EP, Flavier S, Christen R. Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol. 2004;54(5):1773–1788. doi: 10.1099/ijs.0.02997-0. - DOI - PubMed
    1. Dikow RB. Genome-level homology and phylogeny of Shewanella (Gammaproteobacteria: lteromonadales: Shewanellaceae) BMC genomics. 2011;12(1):1. doi: 10.1186/1471-2164-12-237. - DOI - PMC - PubMed
    1. Bowman JP, McCammon SA, Nichols DS, Skerratt JH, Rea SM, Nichols PD, McMeekin TA. Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5w3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol. 1997;47:1040–1047. doi: 10.1099/00207713-47-4-1040. - DOI - PubMed

LinkOut - more resources