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. 1979 Jul;76(7):3338-42.
doi: 10.1073/pnas.76.7.3338.

Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium

M Taub, L Chuman, M H Saier Jr, G Sato

Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium

M Taub et al. Proc Natl Acad Sci U S A. 1979 Jul.

Abstract

Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate substitutes for prostaglandin E1 in the medium. Potential applications of the serum-free medium are discussed. The medium permits a defined analysis of the mechanisms regulating hemicyst formation by hormones and permits the growth of primary kidney epithelial cell cultures in the absence of fibroblast overgrowth.

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References

    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5046-50 - PubMed
    1. Biochem Biophys Res Commun. 1977 Jun 20;76(4):1181-7 - PubMed
    1. Annu Rev Physiol. 1977;39:97-133 - PubMed
    1. Exp Pathol (Jena). 1976;12(3-4):218-22 - PubMed
    1. J Cell Biol. 1972 Mar;52(3):559-68 - PubMed

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