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. 2014 May 5;4(9):e1120.
doi: 10.21769/BioProtoc.1120.

Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

Alexandre Calon  1 , Elisa Espinet  1 , Sergio Palomo-Ponce  1 , Daniele V F Tauriello  1 , Mar Iglesias  2 , María Virtudes Céspedes  3 , Marta Sevillano  3 , Cristina Nadal  4 , Peter Jung  4 , Xiang H-F Zhang  5 , Daniel Byrom  6 , Antoni Riera  6 , David Rossell  7 , Ramón Mangues  7 , Joan Massague  7 , Elena Sancho  7 , Eduard Batlle  7
Affiliations

Immunostaining Protocol: P-Stat3 (Xenograft and Mice)

Alexandre Calon et al. Bio Protoc. .

Abstract

We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells compared with those derived from control cells. STAT3 signaling depended on GP130 as shown by strong reduction of epithelial p STAT3 levels upon GP130 shRNA-mediated knockdown in CRC cells.

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Figures

Figure 1
Figure 1. p-STAT3 staining of liver metastases generated after intrasplenic injection of CRC cells
Note strong staining in epithelial cells (arrows). E: epithelial cells, Str: stromal cells. Scale bar = 50 μm
See this image and copyright information in PMC

References

    1. Calon A, Espinet E, Palomo-Ponce S, Tauriello DV, Iglesias M, Cespedes MV, Sevillano M, Nadal C, Jung P, Zhang XH, Byrom D, Riera A, Rossell D, Mangues R, Massague J, Sancho E, Batlle E. Dependency of colorectal cancer on a TGF-beta-driven program in stromal cells for metastasis initiation. Cancer Cell. 2012;22(5):571–584. - PMC - PubMed